Figure 3.

Determination of plasma amylase and lipase activities in mice. Experimental AP model was induced by hourly intraperitoneal injections of cerulein (50 μg/kg × 6) to mice. Then the HRS at various doses or control saline (CS) was injected intraperitoneally to AP mice 1 h after the final injection of cerulein, respectively. Animals in each group (n = 6) were sacrificed by CO₂ asphyxiation 1 h after the final administration of HRS, and immediately the blood and tissue samples were collected. The amylase and lipase activities in plasma were determined with a colorimetric method using the commercial kits for amylase and lipase, respectively. L: low dose (2 mL/kg × 3); M: middle dose (4 mL/kg × 3); H: high dose (8 mL/kg × 3).