Figure 4.

Histopathological examination of pancreatic tissue from mice. The HRS at various doses or control saline (CS) was injected intraperitoneally to AP mice 1 h after the final injection of cerulein, respectively. One hour later, animals in each group (n = 6) were killed and the pancreatic tissue samples were immediately collected. After fixed in formaldehyde and embedded in paraffin, the samples were sectioned and stained with hematoxylin and eosin, using a standard staining procedure. Histopathological evaluation was performed under light microscope and the scores were calculated according to histopathological changes including edema, inflammatory infiltration, hemorrhage, and necrosis. (a) Images of histological sections; I: sham; II: AP model group; III: AP + CS; IV: AP + HRS (L); V: AP + HRS (M); VI: AP + HRS (H); scale bars = 50 μm. Significant histopathological changes like cytoplasmic vacuole generation, inflammatory cells infiltration, and parenchyma necrosis were indicated by blue arrows. (b) Histopathological scores. *P < 0.05, compared with AP group.