FIG. 5.

SQLs act after virus entry but before integration. For all Q-PCR experiments, cells were treated either with 25 μM FZ41 (white bars) or 25 μM L-731988 (gray bars) inhibitors or not treated (controls; black bars). (A) Quantitative RT-PCR was used to determine the amount of genomic RNA that entered the cells. Dextran sulfate was used as a control inhibitor for unspecific virus entry. Cells were treated with FZ41 or 8 μg of dextran sulfate/ml. (B) Amount of HIV DNApresent at the end of reverse transcription (RT), as determined by R-Gag Q-PCR. (C) Amount of HIV-integrated DNA, as determined by Alu-LTR Q-PCR.