FIG. 1.

Actin depolymerization complements the infectivity defect of ΔNef virions. Magi+/+ cells were infected in the presence of 3 μM CytD, 5 μM LatB, or dimethyl sulfoxide without drug (−) as a negative control. Infection was quantified using a liquid β-Gal assay (A) or by fixing infected cells and counting the number of β-Gal-positive infected foci (B). (C) Cells were infected in the presence of 125 nM Jas or in the absence of Jas (−) as a negative control, and β-Gal expression was quantified using a liquid β-Gal assay. Bold numbers above the bars represent the fold increase in infection relative to the no-drug infection (−) (negative control). OD 405 nm, optical density at 405 nm.