FIG. 4.

Actin depolymerization complements the infectivity defect of ΔNef virions independently of coreceptor tropism and in other viral strains. (A) Cells were infected with equivalent amounts of WT and ΔNef viruses in which the envelope has been deleted that were pseudotyped with the JRFL envelope protein in the presence of 3 μM CytD or in the absence of drug (−) as a negative control. Infection was measured 36 h p.i. using a fixed cell β-Gal assay. (B) Cells were also infected with equivalent amounts of NL43 and NL43ΔNef in the presence of 3 μM CytD, 5 μM LatB, or dimethyl sulfoxide (negative control) (−). Infection was assayed using a liquid β-Gal assay. OD 405nm, optical density at 405 nm.