FIG. 6.

Actin depolymerization complements the infectivity defect of ΔNef HIV-1 virions in GHOST cells. GHOST cells were infected in duplicate in the presence of 3 μM CytD or 5 μM LatB or in the absence of drug (−) as a negative control. Cells were fixed in 1% paraformaldehyde and analyzed for GFP expression following infection with WT LAI and LAIΔNef virions (A) or Env-deficient versions of WT and ΔNef virions pseudotyped with VSV-g envelope protein (B) or amphotropic MLV envelope protein (C). The bars represent the mean percentages of infected cells of duplicate infections determined by analyzing more than 5,000 cells/infection.