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Schematic of the galactosyltransferase gene GGTA1 and replacement vector pGalGTΔS-loxNeo. The genomic organization of the region of the genomic GTTA1 allele targeted by the GTTA1 replacement vector pGalLoxNeo is shown. Primer locations used for assessment of the 5′ and 3′ targeting events as well as the diagnostic restriction sites are presented. Note that integration-specific PCRs are achieved by amplification across the genomic DNA-to-vector boundaries.
