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Replication competence of PERV released from engineered miniature swine PED cells. The replication competence of the ecotropic virus produced by miniature swine PED cells was assessed by using PERV infectivity assays in Gal-positive wild-type ST-IOWA+/+ (A) and Gal-null ST-IOWA−/− (B) target cells. The inoculating virus was produced from either PED+/+ (▵), PED+/− (○), or PED−/− (□) cells, and infection was monitored by RT assay.
