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. 2004 Jun;78(11):5679–5685. doi: 10.1128/JVI.78.11.5679-5685.2004

FIG. 3.

FIG. 3.

Analysis of promoter strength in mammalian BSR-T7/5 or mosquito C6-IBT7/3 cells. Cells were transfected with pTM1-based (for mammalian cells) or pT7Ac-based (for mosquito cells) L and N expression vectors and the appropriate minigenome plasmid vector pT7riboBUNMREN(−), pT7riboBUNLREN(−), pT7riboBUNSREN(−), or pT7riboBUNSREN(−)mut16. pT7AcLuc was cotransfected as an internal control to standardize luciferase activities. (−) control, negative control, showing background minigenome activity in the absence of functional L protein. Activities are expressed in light units or as fold induction of Renilla luciferase (Ren luc) activity.