FIG. 2.
Digital images of rabbit polyclonal antilamin A/C staining of HEp-2 cells that were mock infected or infected with HSV-1(F) (WT), UL31 null mutant (31−), UL34 null mutant (34−), or a virus bearing a restored UL34 gene (34R). Cells were infected at an MOI of 5. At 16 h postinfection (h.p.i.), cells were fixed and permeabilized with ice-cold methanol and incubated with rabbit polyclonal antiserum recognizing lamin A/C and ICP4 monoclonal antibody to mark infected cells. Bound antibodies were detected with Alexa 488 fluorophore-conjugated goat anti-rabbit antibody (yielding a green signal for positive lamin staining) and Alexa 568 fluorophore-conjugated goat anti-mouse secondary antibody (yielding a red signal for positive ICP4 staining).