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Function of APOBEC proteins in viral replication. APOBEC proteins were coexpressed with wild-type or Vif-defective HIV in 293T cells. After normalization by p24Gag enzyme-linked immunosorbent assay, equal amounts of viruses were used to infect GHOST-R3/X4/R5 cells. The expression of Vif in cell lysates from wild-type or Vif-defective viruses was demonstrated by Western blotting (bottom).
