FIG. 5.

Validation of microarray data by protein level and comparison between MVA and WR infections. Shown are Western blots of different cellular proteins at various times (2, 6, and 16 h postinfection). (A) Actin and tubulin protein levels in HeLa cells (5 PFU/cell). (B) RAC-1, WAVE, and NF-κB protein levels in MVA- or WR-infected HeLa cells (5 PFU/cell) and in cells infected with MVA in the presence of cycloheximide (CHX; 100 μg/ml) or cytosine arabinoside (ARAC; 50 μg/ml) for 16 h. (C) Levels of IL-6 and IFN-γ secreted from HeLa cells after MVA or WR infection (5 PFU/cell), as determined by ELISA. Protein levels of IL-6 and IFN-γ in supernatants of uninfected and MVA- or WR-infected cells were measured at 2, 6, and 16 h postinfection. Duplicate samples were measured in two independent experiments. Kd, kilodaltons