TABLE 3.
Confirmation of microarray data by quantitative real-time RT-PCRa
| Gene product | Change (fold) determined by assay at time (h) postinfection
|
|||||
|---|---|---|---|---|---|---|
| Microarray
|
RT-PCR
|
|||||
| 2 | 6 | 16 | 2 | 6 | 16 | |
| H2BFB | 9.92 | 10.20 | 17.03 | 25 | 18 | 19 |
| PCNT2 | 3.94 | 3.14 | 2.17 | 4.36 | 4.44 | 3.4 |
| WASF1 | 3.01 | 2.66 | 1.11 | 3.73 | 2.72 | 1.53 |
| WAS | 2.58 | 2.25 | 1.61 | 5.08 | 15.2 | 2.3 |
| IL7 | 6.10 | 5.30 | 3.2 | 6.86 | 5.8 | 4.9 |
| IL6 | 2.45 | 3.02 | 1.90 | 1.75 | 2.07 | 2.28 |
| IFNG | 1.27 | 1.36 | 1.45 | 1.35 | 1.15 | 1.19 |
| APEXL2 | 1.10 | 0.98 | 1.11 | 0.96 | 1.2 | 0.84 |
| FLJ | 0.98 | 1.25 | 1.01 | 1.1 | 1.02 | 0.93 |
a
RT-PCR conditions are described in Materials and Methods.