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. 2004 Jun;78(11):5670–5678. doi: 10.1128/JVI.78.11.5670-5678.2004

FIG. 1.

FIG. 1.

Generation of the infectious chimeric virus MHIV-mMA12CA. (A) Schematic representation of genomic organization of HIV-1, MLV, and MHIV (upper). Protein structure of the gag gene of these viruses is also shown (lower). MHIV-mMAp12CA encodes the MLV MA, p12, and CA and all the HIV-1 genes other than the HIV MA and CA. (B) Western blot analysis of purified virus particles of MHIV-mMA12CA together with HIV-1 and MLV. Polyprotein processing was tested for HIV-1 MA (p17gag) and CA (p24gag) as well as MLV MA (p15gag) and CA (p30gag) in addition to HIV-1 IN. Some unprocessed Gag and Gag-Pol proteins remain in the lane of MHIV-mMA12CA, but the anti-IN profile of MHIV-mMA12CA is the same as that of parental HIV-1 (not shown in this figure). (C) Single-cycle infectivity of MHIV-mMA12CA. Single-cycle infections of MHIV-mMA12CA were tested either alone or with envelope expression of VSV-G or a truncated HIV-1 Env in trans. Infectivity was measured with the MAGI assay by counting β-galactosidase-positive cells 2 days postinfection using viruses harvested after transfection of proviral clones with or without env expression vectors into 293T cells. These data are representative of three independent experiments.