FIG. 4.

Suppression of cytokine expression by HSV-1 is independent of PKR. RAW264.7-derived cell lines stably transfected with control plasmid (pBK CMV) or a dominant-negative PKR construct (PKR M7) were serum starved for 5 h before treatment with a mock preparation or infection with wt HSV-1 or the mutant in1814 (VP16), vi-13 (ICP4), or d27-1 (ICP27) (MOI of 1). Supernatants were harvested 24 h later, and IL-6 was measured by ELISA. The results are shown as means of triplicate cultures ± SEM. Essentially similar results were observed in three independent experiments.