TABLE 1.
Intermolecular competition between unmarked circular genomic templates and marked linear dimeric RNA
| Source of unmarked circular monomersa | Ratio of unmarked to marked RNAs before and after replication was initiatedb at day:
|
|||
|---|---|---|---|---|
| 0 | 4 | 6 | 9 | |
| Genomic RNA from HDV particles | 1.5 | 6.8 | 7.0 | 6.9 |
| Total RNA from transfected Huh7 cells | 0.16 | 1.0 | 0.6 | — |
a
The two sources of circles are described further in the text.
b
In Northern analyses of the RNAs from the cotransfected δ293 cells, two sequence-specific oligonucleotide hybridizations were carried out, as in Fig. 2. Quantitation for the marked and unmarked RNAs was carried out by using a bio-imager (Fuji) with results as shown. —, not detected.