FIG. 7.

Association of Hsc70 with VLPs. Pseudovirions and VLPs were extracted from cells expressing L1 either alone or together with wild-type or mutant L2 and were subsequently purified by cesium chloride density gradient centrifugation. Fractions containing pseudovirions or VLPs were subjected to centrifugation through linear sucrose gradients. Fractions were analyzed by SDS-PAGE and immunoblotting for L1, L2, Hsc70, and Hsp40 as indicated. For detection of L2, Hsc70, and Hsp40, a fourfold-greater amount of protein was used than for L1, as well as longer exposure to film. Sedimentation was from left to right. Fraction numbers are given above the top gel.