FIG. 7.
Concentration of CypA into Gag particles. Donor membranes (DM) and Gag particles (g-p) were produced by using SFV-C/Pr55gag vector-infected BHK-21 cells that were pre- and postlabeled with [35S]Met but with cyclosporine (Cs) added at the indicated concentrations at 2 h postinfection. Gag particles (lanes 4 to 9) and donor membranes (lanes 3 and 10) were adjusted to contain equal amounts of membranes and then analyzed by SDS-PAGE (6 to 15%) in duplicate. One gel was processed for autoradiography (A), and the other one was used for the detection of CypA by Western blotting (B). Donor membranes from uninfected cells were analyzed in parallel (lane 2). Std, standard.