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. 2014 Aug 24;2014:485743. doi: 10.1155/2014/485743

Figure 5.

Figure 5

Synergistic effect of PAP and CD73 deficiency on the leukocyte subpopulations. Lymphocyte phenotypes in blood (a), spleen (b), lymph nodes (c), and thymus (d) of wild type mice (n = 7), CD73 single (n = 3), PAP single (n = 6), and CD73/PAP double knockouts (n = 4) (thymus: n = 3 for all genotypes) were analyzed using flow cytometry. (e) Regulatory T cells in lymph nodes. Examples of the FACS analyses of regulatory T cells in wild type and CD73/PAP KO mice are shown. In the left panels the gating strategy is shown and in the middle panels percentages of CD4+/CD25+ cells are indicated. Intensities of FoxP3 stainings of CD4+/CD25+ cells are shown as histograms in the right panels. Logarithmic fluorescence is on x-axis and the number of cells on y-axis. (f) Combined results of regulatory T cell analyses of different organs are shown as indicated.  *P ≤ 0.05,  **P ≤ 0.01, and  ***P ≤ 0.001.