Figure 2.

Intracellular Ca²⁺ measurement (using Fura-2-AM) in macrophages subjected to treatment with the P2X7 agonist BzATP and/or with the antagonist A740003. (a) Changes in intracellular Ca²⁺ levels in macrophages from control (black line) and S. mansoni-infected (red line) mice. Inset: representative images from macrophages (control) before (baseline) and after treatment with 100 μM BzATP. (b) Changes in intracellular Ca²⁺ levels ([Ca²⁺]_i) in macrophages from control (white bars) or S. mansoni-infected (black bars) mice. Three plates were used for each condition/animal, 10 cells/plate were chosen randomly for imaging, and cells were obtained from three animals. Data were expressed as mean and SEM. *P < 0.05; ***P < 0.001 (one-way ANOVA followed by post hoc Newman-Keuls test).