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. 2014 Aug 24;2014:134974. doi: 10.1155/2014/134974

Figure 5.

Figure 5

P2X7 receptors expression in peritoneal macrophages. (a) Macrophages from uninfected (white bar) and S. mansoni-infected (black bar) mice express similar levels of P2X7 protein. n = 9 replicates from three different animals, for each group. The images above are representative Western blots (U = uninfected and I = infected). (b) P2X7 receptors expression in peritoneal macrophages from control mice (white bar, untreated) or TGF-β1-treated cells (5 ng/mL, 24 h; black bar) are also similar. The images above are representative Western blots (U = uninfected; TGF-β1= TGF-β1-treated macrophages). Lysates from P2X7 KO macrophages were used as negative controls for P2X7 protein expression. Cells lysates were obtained from three animals for each group. (c) Quantitative RT-PCR (qRT-PCR) analysis of P2X7 receptor mRNA levels in peritoneal macrophages. Similar levels of P2X7 mRNA were found in uninfected (white bar), S. mansoni-infected mice (black bar), and TGF-β1-treated (5 ng/mL, 24 h; hatched bar) mouse macrophages. N = 3–5 samples per group (using different animals).