Figure 5.

Downregulation of Lhx2 by siRNAs enhances RANKL-induced osteoclast differentiation. (a and b) BMMs transfected with control or Lhx2 siRNAs were cultured with M-CSF and increasing concentrations of RANKL for 4 days. (a) Cells were fixed and stained for TRAP. (b) Numbers of TRAP-positive multinucleated cells per well were counted. Data represent means±S.Ds. ^#P<0.05, **P<0.001 versus control. (c) BMMs transfected with control or Lhx2 siRNAs were cultured with M-CSF and RANKL for 2 days. Quantitative real-time PCR was performed for the mRNA expression of Lhx2, NFATc1, TRAP, and OSCAR. Data represent means±S.Ds. ^#P<0.05, *P<0.01, **P<0.001 versus control