Skip to main content
. 2014 Jun 6;21(10):1633–1641. doi: 10.1038/cdd.2014.74

Figure 2.

Figure 2

Nupr1 silencing triggers OIS in human pancreatic cancer cells. (a) Western blot and RT-qPCR of Nupr1 showing the effectiveness of specific siRNA in MiaPaCa2 cells. (b) Cytometric cell cycle analysis showing increase of G1-arrested cells by siNupr1 treatment in MiaPaCa2 cells. (c) MiaPaCa2 cell proliferation curve shows a significant stop in cell proliferation after siNupr1 silencing compared with siControl-treated cells. The relative cell number at each time point on the growth curves represents the mean±S.D. of triplicate normalized to the cell number at day 1. (d) SA-βGal staining of MiaPaca2 cells. A very significant increase of senescent cells is observed after Nupr1 depletion. (e) SA-βGal activity staining in Panc1, CaPan-2 and BxPC-3 cells treated with a siRNA against Nupr1. (f) Bright field and β-tubulin immunofluorescence of MiaPaCa2 cells treated with siNupr1 or siControl. Change in cell morphology and significant increase in cell size is observed in Nupr1-silenced cells. (g) Vimentin immunofluorescence showing significant increase of focal adhesions number in siNupr1-treated cells. Error bars±S.D.; *P<0.05, **P<0.01 and ***P<0.001. Scale bar, 10 μm