Figure 7.

sLZIP enhances bone mass in vivo. (a) Hematoxylin/eosin staining of femurs in WT (n=6) and sLZIP TG (n=5) mice. (b) Histomorphometric analyses of bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular thickness (Tb. Th.), bone number (Tb. N.), and separation (Tb. Sp.) in WT (n=6) and sLZIP TG (n=5) mice. *P<0.001, **P<0.005. (c and d) Micro-CT analysis of trabecular bones in femurs of 10-week-old WT (n=6) and sLZIP TG (n=5) mice. Two-dimensional (c) and three-dimensional (d) micro-CT analyses of distal part of femur and proximal part of tibia. (e) Cross-sectional images of the cortical bone. (f) Alizarin Red and Alcian Blue staining of mouse embryos at E17.5 stage (n=5) (Cr: cranium, Mx: maxilla, Md: mandible, St: stylopod, Ze: zeugopod)