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. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: J Thromb Haemost. 2013 Jul;11(7):1374–1384. doi: 10.1111/jth.12275

Figure 6. Factor IX with fVII sequence in the Gla-domain.

Figure 6

(A) Primary sequences of the human factor IX (fIX) and factor VII (fVII) Gla-domains. The numbering system shown is for fIX. The symbol γ indicates the position of γ-carboxyglutamic acid residues. Underlined sequences were changed from fIX sequence to fVII sequence to generate chimeras C1, C2, C3 and C4. The amino acids changed in each chimera are highlighted by the gray boxes. (B) Stained SDS-polyacrylamide gel of purified plasma fIX (pIX), recombinant wild type fIX (WT), fIX/fVII chimeras (C1, C2, C3, and C4) and fIX in which the Gla-domain from residues 1 through 46 are changed to fVII sequence (fIX/VII-Gla - abbreviated VIIGla). The weaker band running underneath fIX C3 is contaminating bovine serum albumin from conditioned media. (C and D) FXIa (2 nM) was incubated with 250 nM fIXWT (○), C1 (●), C2 (□), C3 (■), C4 (Δ) and fIX/VII-Gla (▲) (250 nM) in TBS with calcium, as described under methods. Shown are concentrations of (C) fIXaβ and (D) fIXα at various times as determined by densitometry of SDS-polyacrylamide gels.