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Ultrathin sections of regenerated nerve stained with toluidine blue at 90 days post-surgery (transmission electron microscope, × 15 000)
(A) Nerve growth factor (NGF)-microspheres group; (B) NGF group, (C) normal saline group; (D) autologous nerve group.
In A and D, myelinated nerve fibers had clear myelin layer structure and uniform size; in B and C, myelinated nerve fibers had disordered myelin layer structure and a lot of fibrous connective tissue.
