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Time course of NKCC1 co-transporter phosphorylation following cSCI. p-NKCC was analyzed on western blots using the anti-p-NKCC antibody R5 and normalized total NKCC1. This value reflects the percentage of NKCC1 phosphorylated. Data are means ± s.e.m., n = 5–6 at sham, acute and chronic phases. One-way ANOVA determined a significant increase in acute (P<0.05) and chronic (P<0.01). Asterisks indicate a significant difference (P<0.05) sham, **= p<0.01, *= p<0.05).
