Fig. 3:

Changes in expression of p-NKCC1 and t-NKCC1 proteins in epicenter during chronic phase of contusion SCI. A. NKCC1 protein expression in epicenter spinal cord tissues of rats (NP = No-Pain and P = pain) following SCI at day 42 (NP) and day 35 (P). Sham (S) samples were obtained from animals subject to laminectomy without subsequent spinal cord contusion. The blot was probed with anti-p-NKCC1 (BL7461 antibody, 1:1000; secondary anti-rabbit 1:2000) and anti-t-NKCC1 (T4 monoclonal antibody, 1:3000; goat anti-mouse 1:3000).