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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Jul 30;1843(11):2620–2630. doi: 10.1016/j.bbamcr.2014.07.012

Fig. 1.

Fig. 1

Bcl3 is highly expressed in CTCL cells, and its expression is inhibited by BZ. Western blotting of whole cell extracts prepared from CTCL Hut-78 (A) and HH cells (B) treated with increasing concentrations of BZ for 24 h, and analyzed by using Bcl3 antibody. To confirm equal protein loading, the membranes were stripped and re-probed with actin antibody. Each lane corresponds to approximately 5 × 104 cells. (C) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 24 h with increasing BZ concentrations. (D) Real time RT-PCR analysis of Bcl3 mRNA levels in Hut-78 cells treated 0, 6, 24 and 48 h with 10 nM BZ. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant (p < 0.05) inhibition compared to control untreated (UT) cells. (E) Western blotting of whole cell extracts prepared from Hut-78, HH, U937, THP1 and PBMC cells analyzed by Bcl3 and control actin antibodies; each lane corresponds to approximately 5 × 104 cells.

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