Fig. 2.
Bcl3 suppression induces apoptosis in CTCL cells. (A) Western blotting of whole cell extracts prepared from Hut-78 cells transfected with control non-silencing and Bcl3 specific siRNA, and analyzed by using Bcl3 and actin specific antibodies. (B) Densitometric evaluation of Bcl3 densities showed in panel A. The Bcl3/actin value in cells transfected with control siRNA was arbitrarily set to 100%, and the Bcl3/actin value in cells transfected with Bcl3 siRNA is presented relative to this value. The data represent the mean of four experiments ± SE, and the asterisk denotes a statistically significant (p < 0.05) change compared to cells transfected with control siRNA. (C) Cell viability and (D) the cytoplasmic nucleosome enrichment assay in Hut-78 cells transfected with control and Bcl3 siRNA. The values represent the mean ± SE of four experiments; the asterisk denotes a statistically significant (p < 0.05) change compared to cells transfected with control siRNA.