Fig. 5.
BZ induces IL-8 and IL-17 expression in CTCL cells. Real time RT-PCR analysis of IL-8 (A) and IL-17 (B) mRNA levels in Hut-78 (full columns) and HH (empty columns) cells treated 24 h with increasing BZ concentrations. ELISA of released IL-8 (C) and IL-17 (D) levels measured in cell culture supernatants of Hut-78 and HH cells treated 24 h with increasing BZ concentrations. Cell viability of Hut-78 cells incubated 24 h with increasing concentrations of human recombinant IL-8 (E) and IL-17 (F) proteins, measured by Trypan Blue exclusion. The values represent the mean ± SE of four experiments. Asterisks denote a statistically significant (p < 0.05) change compared to untreated cells.