Skip to main content
. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: Nat Chem Biol. 2014 May 11;10(6):470–476. doi: 10.1038/nchembio.1525

Figure 1. Production and validation of MGM and recognition of microbial glycan structures by sera.

Figure 1

(a) Schematic of MGM design and utility. (b) MGM data obtained after incubation with pooled normal human sera at 1:1000 followed by labeled anti-IgG and anti-IgM). b inset: magnification of antibody interactions with increasing printed concentration of Providencia stuartii O18 BPS (31.25, 62.5, 125, 250, 500 µg/mL). (c) MGM probed with 200 µg/ml IVIG pooled from ~10,000 human donors followed by detection with anti-IgG. (d,e) Detection of pooled normal mouse sera at 1:1000 (d) or pooled normal rabbit sera at 1:1000 (e) with anti-mouse or anti-rabbit IgG, respectively. (f) MGM data obtained after incubation with 1:5000 dilution of sera from rabbits challenged with the indicated bacterial species followed by detection with anti-rabbit IgG. See Supplementary Dataset 1 for complete microarray data.