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. Author manuscript; available in PMC: 2014 Sep 9.
Published in final edited form as: Nat Chem Biol. 2014 May 11;10(6):470–476. doi: 10.1038/nchembio.1525

Figure 4. Galectins bind and kill a broad range of new bacterial targets.

Figure 4

(a–c) CFGv4.2 glycan microarray assayed with 0.2µM Gal-4 (a), 5µM Gal-4 (b), and 5µM Gal-8 (c). Error bars represent means ± 1 s.d. See Supplementary Dataset 2 for complete microarray data. (d) Flow cytometric analysis of S. marcescens O20 (SM O20) after incubation with ~0.1µM Gal-4 with or without inclusion of 20mM TDG. (e) Quantification of SM O20 after addition of 5µM Gal-4 with or without addition of 50mM TDG or sucrose (Sucr). (n=2–3 in 1 representative experiment of 3). (e) Flow cytometric analysis of H. influenzae 2019 (NtHi 2019) after incubation with ~0.1µM Gal-8 with or without inclusion of 20mM TDG. (f) Quantification of NtHi 2019 after addition of 5µM Gal-8 with or without addition of 50mM TDG or Sucr. (n=2 in 1 representative experiment of 3). (g) Quantification of Streptococcus agalactiae A909 (GBS A909) after addition of 5µM Gal-8 with or without addition of 50mM TDG or Sucr.