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. 2014 Apr 4;28(9):1811–1818. doi: 10.1038/leu.2014.100

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Copyright © 2014 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Immunostaining of bone marrow biopsies with the anti-mutated CALR antibody. Panel a shows two megakaryocytes labeled by the anti-mutated calreticulin antibody together with a negative one. In panel b, abnormal, small megakaryocytes in the bone marrow of a CALRins5 PMF patient are shown. Panel c shows a low-resolution picture of an advanced fibrosis in a CALRdel52 patient and d is a high-power field from panel c (square) to show the abnormally shaped large megakaryocytes within buddles of fibers. In panels e and f, the faint label of erythroid and myeloid cells is presented at higher magnification.