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Immunohistochemistry for GAD65. (A–L) Representative images of RZ, RZ+FS and RZ+FS+MC, 1, 4, and 8 weeks post lesion. (M) Quantification (ratio IL/CL) of the integrated density of pixels, obtained in the region delimited by dashed lines. (N) Representation of lamina III (total quantification area 3.9 × 105 μm2). CL, contralateral, FS, fibrin sealant; IL, ispsilateral; MC, mononuclear cells; RZ, rhizotomy. Scale bar = 50 μm. *p < 0.05; **p < 0.01.
