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Immunohistochemistry for glial fibrillary acidic protein (GFAP). (A–L) Representative images of RZ, RZ+FS and RZ+FS+MC, 1, 4, and 8 weeks post lesion. (M) Quantification (ratio IL/CL) of the integrated density of pixels, obtained in the region delimited by dashed circles. (N) Representation of lamina IX (total quantification area: 8.5 × 105 μm2). CL, contralateral; FS, fibrin sealant; IL, ispsilateral; MC, mononuclear cells; RZ, rhizotomy. Scale bar = 50 μm. **p < 0.01; ***p < 0.001.
