Fig. 2.

Activation of WT AMPK and the AMP/ADP-insensitive RG mutant by GO and H₂O₂. (A) Activation of AMPK in response to GO (10 mU/ml) measured in anti-FLAG immunoprecipitates in WT and RG cells. Results are mean ± SEM (n = 20–24). (B) As (A), but adding a single dose of H₂O₂ at the calculated final concentration shown, and then preparing cell lysates 60 min later. Results are mean ± SEM (n = 4). (C) As (B), but varying the time of incubation with a calculated final concentration of 1 mM H₂O₂. Results are mean ± SEM (n = 4). Statistical significance was assessed by 1-way ANOVA using Sidak’s multiple comparison test: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.