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Identification of a SUMOylation site in IRF8. The 293T cells transfected with Flag-IRF8 mutants (K72R or K310R) and T7-SUMO1 or V5-SUMO3 were immunoprecipitated with anti-Flag Ab and blotted with anti-T7 and anti-V5 Ab (upper middle panel) or anti-Flag Ab (lower middle panel). Vector alone and wt Flag-IRF8 (wt) were tested as controls. The bottom panel indicates immunoblot analysis of WCE.
