FIG. 3.

Chloroplast localization of AtTPPD. (A) Subcellular localization of AtTPPD protein in leaves of Arabidopsis thaliana plants stably transformed with 35S::AtTPPD-CFP. Ten-day-old seedlings were analyzed by confocal microscopy. The CFP signal (blue) is shown in the first channel and chlorophyll autofluorescence (red) in the second. The third channel shows the merged image. The scale bars represent 10 μm. (B) Western blot analysis of A. thaliana leaf total protein and purified chloroplast extracts from 4-week-old wild-type (Col-0) and AtTPPD overexpressor line (AtTPPD OE). AtTPPD-CFP proteins were detected with an anti-GFP antibody. The photosystem II protein D1 antibody (PsbA/D1) was used as a chloroplast marker. CBB, Coomassie Brilliant Blue.