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. 2014 Jul 22;6(8):1911–1937. doi: 10.1093/gbe/evu151

Table 2.

Overview of miRNAs Characterized in Teleosts

Developmental Stage/ Tissue/Process miRNA Species Method Function References
Embryonic development miR-430 Zebrafish Cloning, knockdown, qRT-PCR Maternal transcript clearance Giraldez et al. (2006)
Gonadal development miR-430 Zebrafish Knockdown, miRNA target protection assays, reporter assays, transgenics, ISH, qRT-PCR PGC migration Staton et al. (2011)
Metamorphosis let-7 Japanese flounder Atlantic halibut NGS and qRT–PCR Larval to juvenile transformation Bizuayehu et al. (2012a) and Fu et al. (2013)
Vascular development miR-126 Zebrafish Microarray, knockdown, luciferase reporter assay, qRT-PCR Angiogenesis Fish et al. (2008)
Vascular development miR-142-3p Zebrafish Knockdown, overexpression, qRT-PCR Vascular integrity, remodeling and angiogenesis Lalwani et al. (2012)
Vascular development miR-221 Zebrafish NGS, ISH, northern blot, qRT-PCR, knockdown, miRNA sensor assays Endothelial tip cell proliferation and migration Nicoli et al. (2012)
Vascular development let-7g, miR-23b, miR-27a, miR-27b, miR-29a, and miR-126 Zebrafish microarray, northern blot, ISH, qRT-PCR, knockdown, luciferase reporter assay Arterial-venous segregation, angiogenesis, branching and tip cell specification Biyashev et al. (2012)
Oocyte and early embryo miR-21, miR-23a, miR-26a, miR-30d, miR-92a, miR-125a, miR-125b, miR-126-5p, miR-126-3p, miR-200b, and miR-455 Rainbow trout Cloning and qRT–PCR ? Ramachandra et al. (2008)
Oocyte and early embryo miR-34 Zebrafish Knockdown, microarray, qRT–PCR Nervous system development Soni et al. (2013)
Oocyte miR-15, miR-29, miR-92, miR-101, miR-126, miR-181-3p, miR-196, miR-202-5p, miR-202-3p, miR-221, miR-301, miR-338, and miR-2184 Rainbow trout Microarray ? Juanchich et al. (2013)
let-7, miR-10, miR-21, miR-24, miR-25, miR-30, miR-143, miR-146, miR-148, and miR-202 Rainbow trout NGS ? Ma et al. (2012)
Brain let-7g, k, h, i, l, miR-29a, b, miR-103, miR-124a, b, c, d, and miR-125 Asian seabass qRT–PCR ? Xia et al. (2011)
let-7a, b, c, and d, miR-9, miR-21, miR-124, miR-135c Zebrafish NGS, qRT–PCR ? Soares et al. (2009)
let-7a,b,c,f,i, miR-7b, miR-9-5p, miR-9-3p, miR-34b, miR-103, miR-107, miR-124a, miR-125a,b, miR-128, miR-129-3p, miR-132, miR-138, miR-181a,b, miR-216, miR-217, miR-219, and miR-375 Zebrafish Microarray, ISH ? Wienholds et al. (2005)
let-7a,b,c, miR-9, miR-34, miR-92b, miR-124, miR-128, miR-135c, miR-137,miR-138, miR-153a, miR-219, miR-222 Zebrafish ISH ? Kapsimali et al. (2007)
miR-7 and miR-9 Zebrafish Gain- and loss-of-function Brain boundary organization Leucht et al. (2008) and Memczak et al. (2013)
Eye miR-124 Zebrafish NGS, qRT–PCR ? Soares et al. (2009)
let-7b, miR-9, miR-30a, miR-92b,miR-96 miR-124, miR-181a,b, miR-182, miR-183, miR-184, and mir-204 Zebrafish ISH ? Kapsimali et al. (2007)
miR-204 Medaka Knockdown, ISH, luciferase reporter assays, qRT-PCR Lens development Conte et al. (2010)
let-7 Zebrafish Luciferase reporter assays, qRT-PCR, knockdown Müller glia cells differentiation Ramachandran et al. (2010)
miR-7 and miR-454a Zebrafish and medaka ISH ? Ason et al. (2006)
miR-7, miR-9, miR-34b, miR-96, miR-124a, miR-125b, miR-132, miR-181b, miR-182, miR-183, miR-184, and miR-204, miR-215, miR-216, miR-217 Zebrafish Microarray, ISH ? Wienholds et al. (2005)
let-7g,n,k,h,i,l, miR-21c, miR-29a,b, miR-124, miR-125, miR-126a,b, miR-181a,b, miR-183a,b, miR-184a,b Asian seabass qRT–PCR ? Xia et al. (2011)
Heart miR-218a-1/2 Zebrafish Knockdown, overexpression, ISH, luciferase reporter assay, qRT-PCR Heart field migration Fish et al. (2011)
miR-138 Zebrafish Knockdown, antagomiR, ISH, luciferase reporter assay, qRT-PCR Cardiac patterning Morton et al. (2008)
miR-21, miR-218a Zebrafish Knockdown, overexpression, ISH, qRT-PCR, luciferase reporter assay Heart valve formation Chiavacci et al. (2012) and Banjo et al. (2013)
let-7e,f,g,h,i,j,k,l,m,n,o, miR-1a, miR-20, miR-21a,b,c, miR-29a,b, miR-103, miR-125, miR-126a,b, miR-128c, miR-145, and miR-199b Asian seabass qRT–PCR ? Xia et al. (2011)
miR-1, miR-101a, miR-130b,c, miR-133a, miR-221, and miR-499 Zebrafish NGS, qRT–PCR ? Soares et al. (2009)
let-7i, miR-15b, miR-17a-3p, miR-21, miR-92b, miR-128, miR-133, miR-146a,b, miR-150, miR-194a, miR-204, miR-210-3p, miR-301a, miR-429, miR-730, miR-733, miR-738, Zebrafish Microarray, northern blot, qRT-PCR, ISH Regeneration Yin, Lepilina, et al. (2012)
Muscle miR-1, miR-21, miR-133a,b,c, miR-203b Zebrafish NGS, qRT–PCR ? Soares et al. (2009)
miR-203b Nile tilapia qRT–PCR and luciferase reporter assay Muscle development Yan, Guo, et al. (2013)
let-7a,c,f, miR-1, miR-17a, miR20a,b, miR-126, miR-133c, miR-181a, miR-203b, miR-206, miR-214, and miR-738 Zebrafish Microarray, northern blotting, qRT–PCR Transition from hyperplasia to hypotrophy Johnston et al. (2009)
miR-499 Zebrafish ISH, overexpression, knockdown, transgenics Muscle fiber-type specification Wang et al. (2011)
miR-140 Zebrafish, medaka Knockdown, reporter assays, cloning, ISH Palatogenesis Ason et al. (2006) and Eberhart et al. (2008)
let-7b,c,j, miR-1, miR-15a, miR-22a, miR-27a, miR-30b, miR-34, miR-125b, miR-133a,miR-140, miR-152, miR-192, miR-193a, miR-199, miR-204, miR-206, miR-214, miR-218a,b, miR-301c, and miR-460 Nile tilapia NGS, qRT-PCR, microarray Growth Huang et al. (2012) and Yan et al. (2012a)
miR-1, miR-21, miR-23a, miR-24, miR-26a, miR-27a,b, miR-29b, miR-125b, miR-133a-3p, miR-155, miR-181a-5p, miR-206, miR-214, miR-221, and miR-222 Common carp NGS, qRT-PCR ? Yan et al. (2012)
miR-1, miR-9b-3p, miR-10b, miR-10d-5p, miR-23b, miR-92a, miR-122, miR-133b-5p, miR-135a,c, miR-144-3p, miR-144-5p, miR-145, miR-193b, miR-212, miR-462, miR-551, and miR-2187-5p Blunt snout bream NGS Growth Yi et al. (2013)
miR-1 and miR-133 Zebrafish Transgenics, microarray, luciferase reporter assay, knockdown, qRT–PCR Actin organization in the sarcomere and its function Mishima et al. (2009)
Fin mi-R2a, miR-26a, miR-66, miR-69, miR-80, miR-144, miR-200b, miR-203, miR-301, and miR-338 Zebrafish Microarrays, reporter assays, knockdown Regeneration Thatcher et al. (2008)
miR-7a, miR-17-5p, miR-20a, miR-21, miR-22a, miR-23, miR-25, miR-26a, miR-31, miR-92b, miR-100, miR-101, miR-124, miR-133,miR-137, miR-138, miR-146, miR-182, miR-187, miR-194b, miR-196a, miR-200a,c, miR-205, miR-216a, miR-301, miR-338, Zebrafish Microarray, northern blot, qRT-PCR, ribonuclease protection assays Regeneration Yin et al. (2008)
Ionocytes, nasal epithelium, neuromasts, pronephros, and scattered epithelial cells miR-8, miR-141, miR-200a, miR-200b, miR-200c, and miR-429 Zebrafish ISH, northern blot, reporter assays Osmoregulation Flynt et al. (2009)
Kidney and gills miR-30c and miR-429 Nile tilapia qRT-PCR, luciferase reporter assay Osmoregulation Yan, Zhao, et al. (2012) and Yan et al. (2012b)

Note.—?, function unknown; ISH, in situ hybridization; NGS, next-generation sequencing; qRT-PCR, quantitative real-time polymerase chain reaction.