Fig. 8.—

Phylogenetic tree and conservation of putative active site residues of BLES03 homologs. (A) Phylogenetic tree of BLES03 homolog. Color on outer ring indicates taxonomic classes of the organism to which the respective protein belongs. To improve clarity, clades containing proteins from same taxonomical classes were collapsed and labeled. The numbers in bracket indicate number of sequences contained in that clade. The numbers next to branches indicate number of time-associated branches clustered together in a bootstrap test with 1,000 replicates. (B) Conservation of probable active site residues of BLES03-like proteins in representative sequences obtained from a sequence similarity search using BLAST. Star numbers are same as figures 4 and 5A. First three sequences are from eukaryotic organism, next two from archaeabacteria, and the last two from bacteria. For clarity few columns of the MSA were hidden, which is depicted by blue vertical lines.