Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 16;17(10):1282–1290. doi: 10.1111/jcmm.12103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

Fig. 1 — (A) Methylation-specific PCR of normal controls using SOCS2-3′ methylation-specific polymerase chain reaction (MSP) primers. M-/U-MSP analysis showed that four of five normal peripheral blood controls (PB1-5) were methylated with the SOCS2-3′ primers. (M: DNA marker; B: blank; PC: positive control; PB, normal peripheral blood control). (B) Sequencing of MSP products using SOCS2-3′ MSP primers in normal controls and positive control showing methylation signals. Methylated cytosine residues [C] in CpG dinucleotide remained as C, whereas unmethylated cytosine read as [T] after bisulphite conversion. (PC: positive control; PB: normal peripheral blood control).