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Methylation-specific polymerase chain reaction of SOCS1, SOCS2-5′, SOCS3, CISH and SHP1 in cell lines. For SHP1, K562 was homozygously methylated, while HEL, SET-2 and MEG-01 were completely unmethylated. HEL, SET-2, MEG-01 and K562 were completely unmethylated for SOCS1, SOCS2-5′, SOCS3 and CISH. (M: DNA marker; B: blank; PC: positive control; N: normal control; C1: HEL; C2: MEG-01; C3: SET-2; C4: K562).
