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. 2013 Oct 16;17(10):1282–1290. doi: 10.1111/jcmm.12103

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© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine

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Fig. 5 — Effect of 5-AzadC treatment on K562 cells. (A) M-/U-methylation-specific polymerase chain reaction analysis of SHP1 promoter methylation status showed that 5-AzadC treatment led to progressive demethylation of SHP1 promoter in K562 cells. (M: DNA marker; B: blank; PC: positive control; N: normal control; D0, day 0; D3, day 3 culture in 5-AzadC with 0.5 μM). (B) Reverse transcription–PCR (RT-PCR) analysis of the GAPDH status and SHP1 expression after 5-AzadC treatment. (M: DNA marker; B: blank; N: normal control; NRT: negative control without reverse transcriptase; D0, day 0; D3, day 3 culture in 5-AzadC with 0.5 μM).