Table 3.
Effects of the study site, sampling season, and sampling year as well as their interactions on soil microbiological properties, as determined by ANOVA (Kruskal-Wallis ANOVA and Mood’s median test were additionally applied for non-parametric data and confirmed the results obtained by ANOVA).
| Parameter | Effect of | Significant effect? | Result | |
|---|---|---|---|---|
|
| ||||
| P-value | yes/no | |||
| RESP | Year | 0.000 | yes | 2010 < 1993 < 2000 |
| Season | 0.000 | yes | Spring > Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.504 | no | ||
| Site * season | 0.681 | no | ||
| Year * season | 0.006 | yes | ||
| Site * year * season | 0.148 | no | ||
| SIR | Year | 0.000 | yes | 1993 < 2000 = 2010 |
| Season | 0.588 | no | Spring = Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.019 | yes | ||
| Site * season | 0.64 | no | ||
| Year * season | 0.001 | yes | ||
| Site * year * season | 0.064 | yes | ||
| DHG | Year | 0.000 | yes | 1993 > 2000 = 2010 |
| Season | 0.670 | no | Spring = Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.000 | yes | ||
| Site * season | 0.761 | no | ||
| Year * season | 0.755 | no | ||
| Site * year * season | 0.224 | no | ||
| XYL | Year | 0.000 | yes | 1993 > 2000 = 2010 |
| Season | 0.000 | yes | Spring < Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.000 | yes | ||
| Site * season | 0.256 | no | ||
| Year * season | 0.000 | yes | ||
| Site * year * season | 0.286 | no | ||
| PROT | Year | 0.000 | yes | 1993 > 2000 = 2010 |
| Season | 0.119 | no | Spring = Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.000 | yes | ||
| Site * season | 0.303 | no | ||
| Year * season | 0.096 | no | ||
| Site * year * season | 0.356 | no | ||
| PME | Year | 0.000 | yes | 1993 < 2010 < 2000 |
| Season | 0.000 | yes | Spring < Autumn | |
| Site | 0.069 | no | IT01 = IT02 | |
| Site * year | 0.159 | no | ||
| Site * season | 0.151 | no | ||
| Year * season | .011 | yes | ||
| Site * year * season | 0.167 | no | ||
| SULF | Year | 0.000 | yes | 1993 < 2010 < 2000 |
| Season | 0.096 | no | Spring = Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.135 | no | ||
| Site * season | 0.972 | no | ||
| Year * season | 0.135 | no | ||
| Site * year * season | 0.799 | no | ||
| NITRIF | Year | 0.205 | no | 1993 = 2000 = 2010 |
| Season | 0.365 | no | Spring = Autumn | |
| Site | 0.614 | no | IT01 = IT02 | |
| Site * year | 0.761 | no | ||
| Site * season | 0.992 | no | ||
| Year * season | 0.210 | no | ||
| Site * year * season | 0.733 | no | ||
| BACTERIA | Year | 0.001 | yes | 1993 < 2000 = 2010 |
| Season | 0.012 | yes | Spring > Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.245 | no | ||
| Site * season | 0.040 | yes | ||
| Year * season | 0.006 | yes | ||
| Site * year * season | 0.656 | no | ||
| FUNGI | Year | 0.000 | yes | 1993 > 2000 > 2010 |
| Season | 0.651 | no | Spring = Autumn | |
| Site | 0.000 | yes | IT01 < IT02 | |
| Site * year | 0.000 | yes | ||
| Site * season | 0.055 | no | ||
| Year * season | 0.055 | no | ||
| Site * year * season | 0.082 | no | ||
The significance level was P<0.05. Abbreviations: RESP, respiration; SIR, substrate-induced respiration; DHG, dehydrogenase; XYL, xylanase; PROT, protease; PME, acidic phosphatase; SULF, sulfatase; NITRIF, nitrification; BACTERIA, bacterial numbers; FUNGI, fungal numbers.