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. Author manuscript; available in PMC: 2014 Sep 26.
Published in final edited form as: J Med Chem. 2013 Sep 4;56(18):7212–7221. doi: 10.1021/jm400474r

Figure 4.

Figure 4

Effects of Cryptotanshinone are decreased in SHP2 knockdown cells. HeLa cells were transfected with control or SHP2 siRNA (400 pmoles/8.0×105 cells). Forty-eight hours later, transfected cells were treated with Cryptotanshinone at the indicated concentrations. Cell numbers were determined 24 hours after the treatment. Cryptotanshinone or DMSO-treated knock-down and control cells were normalized against untreated knock-down and controls cells, respectively. SHP2 levels in knockdown and control cells were analyzed by immunoblotting analyses with anti-Shp2 antibody. Blots were stripped and reprobed with anti β-actin antibody to monitor protein loading. Experiments were performed three times. Similar results were obtained in each. Data shown are mean±S.E.M. of triplicates from one representative experiment.