Skip to main content
. 2014 Sep 9;9(9):e106250. doi: 10.1371/journal.pone.0106250

Figure 1. Quantification bias in the assessment of BCR-ABL1 transcript levels by using ABL1 as control gene.

Figure 1

Localization of primers (arrows) and probes (rectangles) used for the relative quantification of the M-BCR-ABL1 transcript according to the EAC protocol [16], [17] are represented. Boxes represented the exon number. Red signs indicate primers and probe relative to the fusion transcript, blue signs to the control gene ABL1. The ABL1 primers localization explains both amplification of ABL1 and BCR-ABL1 during the Q-RT-PCR reaction. ENF = forward primer, ENPr = TaqMan reverse probe, ENR = reverse primer.