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. 2014 Sep 9;9(9):e106112. doi: 10.1371/journal.pone.0106112

Figure 3. Kank can localise transiently to the nucleus of S2 cells.

Figure 3

(A) Kank(1–500) and Kank(889–1224) exhibit nuclear localisation, while truncations of Kank which contain the middle region do not. S2 cells were transfected with GFP-fused Kank truncations. Cells were then co-stained for GFP, α-tubulin and DNA. (B) Kank shuttles between the nucleus and the cytoplasm in some cells. S2 cells transfected with GFP-Kank were incubated with media containing leptomycin B for 3–3.5 hours, to inhibit nuclear export. Control cells were incubated with media containing methanol, the solvent for leptomycin B. Nuclear localisation was observed more frequently in leptomycin B treated cells than control cells. Significance was determined by Fishers exact chi squared test. Error bars show the 95% confidence interval. (C) A summary of Kank truncations and their nuclear localisation. + and – indicate the presence and absence of the nulcear localisation with (+LB) or without (–LB) Leptomycin B. ND (Not done). Scale bars = 5 µm.