Figure 5.

FH1-4-C3b co-crystal structure demonstrating the position of the R53C and D90G mutations. An X-ray-derived co-crystal structure of FH-C3b (23) was used to model the mutation and displayed with Pymol (Delano Scientific). The location of the two mutated amino acids (red spheres) are shown within the co-crystal structure of a FH1-4 (dark grey):C3b (light grey) complex. The R53 amino acid is on the opposite face to the FH-C3b interface, while the D90 amino acid is in direct opposition to C3b (Protein Data Base ID code 2WII) (23).