Figure 5.

Both the agenet and KH domains are important for dFMR1 to associate with chromatin. (A) Schematic drawings of dFmr1 and its deletion mutants. (B and C) S2 cells expressing Flag-dFmr1 ^ΔKH1 (B) or Flag-dFmr1 ^ΔAgenet (C) without treatment (left) or treated with both HU and LMB (right) were stained with anti-Flag (green), anti-dFMR1 (red) antibodies and Hoechst (blue). (D) S2 cells expressing the indicated proteins were irradiated with or without UV, and the chromosome fractions were separated. Western blots with anti-FLAG, anti-GAPDH, anti-H2B and anti-α-tubulin antibodies were performed to show the levels of indicated proteins.